Egfl7 与非小细胞肺癌上皮间质转化关系的研究

目的:研究Egfl7与非小细胞肺癌(NSCLC)上皮间质转化标志物E-cadherin,Vimentin的相关性,探讨Egfl7是否参与NSCLC的上皮间质转化(EMT)。方法:分别采用免疫组化法和RT-PCR法检测40例NSCLC组织和20例肺癌旁正常肺组织中Egfl7,E-cadherin和Vimentin蛋白和mRNA的表达情况。结果:1).NSCLC组织中的Egfl7蛋白和mRNA的表达水平明显高于癌旁正常肺组织;其差异有统计学意义(P<0.05)。Egfl7的表达水平与肺癌的临床分期、及淋巴结转移密切相关(p0.05)。结论:NSCLC组织中Egfl7高表达,Egfl7可能与NSCLC的侵袭性相关;Egfl7与E-cadherin呈负相关,与Vimentin表达成正相关,Egfl7可能参与了NSCLC患者的上皮间质转化(EMT)过程,阻断Egfl7信号可能会抑制NSCLC患者的ENT。

Relationship of Egfl7 and Epithelial-Mesenchymal Transition in Patients with Non-small Lung Cancer

Objective: To study the relationship of Egfl7 and E-cadherin,Vimentin in epithelial-mesenchymal transition(EMT) and to investigate whether of Egfl7 involved in EMT progress in the patients with non-small cell lung cancer(NSCLC).Methods: Forty NSCLC and Twenty normal Pulmonary tissues were obtained Immunonistochemistry and RT-PCR were performed to determine the expr-essions of Egfl7,E-cadherin and Vimentin protein and mRNA in the NSCLC tissues.To explore the correlation between Egfl7 expression levels and EMT of NSCLC patients.Results: 1.The result of Immunohistechermial and RT-PCR analysis indicate the expression level of Egfl7 protein and mRNA were higher in NSCLC tissues than those in normal(P<0.05).Our data also indicate that there is a highly signifi-cant correlation of the total Egfl7 protein and mRNA expression levels with clinical degree and Lymph node metastasis(P<0.05).2.The expression rate of E-cadherin protein and mRNA were significantly lower in NSCLC than those in normal.The expression of E-cadherin in metastasis group of 40 NSCLC was lower than metastases group.3.The expression rate of Vimentin protein and mRNA were higher in NSCLC than those in normal.The expression of Vimentin in metastasis group of 40 NSCLC was higher than metastases group.4.The ex-pression of Egfl7 was negatively correlated to that of E-cadherin(r=-0.34,P<0.05),but positively correlated to that of Vimentin(r=0.297,P<0.05);in addition,the expression of E-cadherin had no correlation with to that of Vimentin(r=0.0002,P>0.05).Conclusions: We firstly demonstrated the high expression of Egfl7 in NSCLC tissues.Egfl7 may be related with aggression of NSCLC;The expression of Egfl7 was negatively correlated to that of E-cadherin,but positively correlated to that of Vimentin.Egfl7 may be involved in EMT progress in the patients with NSCLC,indicating that a blockage of Egfl7 may suppress EMT in NSCLC.