阿司匹林对去势大鼠腰椎骨密度及微观结构的影响

目的:研究阿司匹林对去势(卵巢切除)大鼠腰椎骨密度及微观结构的影响。方法:取48只3月龄SD雌性大鼠随机分为6组:去势组(OVX组)、对照组(Sham组)及4个阿司匹林治疗组(Aspirin组),每组8只。OVX组及Aspirin组采用卵巢切除法建立骨质疏松模型。去势后1周,阿司匹林治疗组剂量分别为2.25、4.46、8.92及26.75 mg/kg(A1、A2、A3及A4组),每天灌胃一次,OVX组及Sham组予同等量生理盐水灌胃。灌胃3个月后处死,剖取腰椎椎体,以双能X线吸收骨密度测量仪(DXA)和Micro-CT进行测量分析。结果:DXA分析结果显示:阿司匹林各剂量组BMD值较OVX组有统计学差异(P<0.01)。Micro-CT分析表明:与OVX组比较,阿司匹林各剂量组BV/TV、Tb.Th、Tb.N、BMD均显著性提高(P<0.01),BS/BV、Tb.Sp显著性降低(P<0.01),阿司匹林各剂量组BV/TV、BS/BV、Tb.Th、Tb.N、Tb.Sp、BMD与Sham组相比有统计学差异(P<0.01)。结论:阿司匹林可以改善去势大鼠骨小梁结构,增加骨质密度,对去势大鼠骨质疏松具有防治作用,其作用途径可能包括抑制骨吸收和刺激骨形成两方面。

The Preventive Effect of Asprin on Osteoporosis in Ovariectomized Rat

Objective: To investigate the effect of aspirin on bone mineral density and microstructure of lumbar vertebra on ovariectomized rats.Methods: 48 three-month-old female SD rats were randomly divided into 6 groups with 8 rats in each: OVX group,Sham group and Aspirin groups(A1 A2 A3 A4),OVX group and Aspirin groups were ovariectomized to establish osteoporosis model,After one week,Aspirin groups was intragastrically administered at a dose of 2.25 mg/kg.d(A1) 4.46 mg/kg.d(A2) 8.926 mg/kg.d(A3) and 26.75 mg/kg.d(A4) daily in OVX rats,OVX group and Sham group were taken the same amount of saline respectively for three months.Then all the rats were excuted and their lumbar vertebras were harvesten.The BMD and structural parameters were measured by dual-energy X-ray absorptiometry and micro-CT respectively.Results: The BMD in Aspirin groups was significantly higher than that in the OVX group(P<0.01).Compared with the OVX group,the BV /TV,Tb.Th,Tb.N,BMD increased significantly in the aspirin groups(P<0.01),While the BS/BV,Tb.Sp is significantly lower(P<0.01).The BV/TV,BS/BV,Tb.Th,Tb.N,Tb.Sp,BMD in the aspirin groups were significantly different compared with that in the Sham group(P< 0.01).Conclusion: Aspirin can improve trabecular bone structure,increase bone mineral density,prevent the occurrence of postmenopausal osteoporosis,and this effect is probably by inhibiting bone resorption and stimulating bone formation.