氯胺酮对抑郁大鼠模型抗抑郁作用研究

目的:探讨在抑郁大鼠模型中单次氯胺酮可产生快速持久地抗抑郁作用。方法:实验一:32只Wistar大鼠随机分为四组(n=8),药物干预前1 d大鼠强迫游泳15 min,药物干预当天,分别腹腔注射相同容积的生理盐水(S组)、5 mg/kg氯胺酮(K5组)、10 mg/kg氯胺酮(K10组)、15 mg/kg氯胺酮(K15组)。30 min后记录大鼠运动能力及不动时间。实验二:20只Wistar大鼠随机分为两组(n=10),所有大鼠均经历21天慢性不可预知应激试验。第22天大鼠分别腹腔注射相同容积生理盐水及10 mg/kg氯胺酮,于干预前、干预后1 h、2 h、6 h、1 d、4 d、7 d分别进行敞箱试验,并记录大鼠水平运动及垂直运动得分。结果:与S组相比,K5、K10及K15组大鼠运动能力无明显变化(P>0.05)且强迫游泳不动时间均显著减少(P<0.01);与干预前生理盐水组相比,生理盐水干预后1 h、2 h、6 h、1 d、4 d及7 d组大鼠敞箱试验水平运动及垂直运动均无明显差异(P>0.05);与干预前氯胺酮组相比,生理盐水干预后1 h、2 h、6 h、1 d、4 d及7 d组大鼠敞箱试验水平运动及垂直运动有明显差异(P<0.05)。结论:在抑郁症大鼠模型中氯胺酮可产生快速且持久的抗抑郁作用。

The Antidepressant Effect of Ketamineon Depressed Rat Model

Objective: To investigate the antidepressant effects of single administration of ketamine on the depressed rat model.Methods: Experiment one: Thirty-two rats were randomly divided into four groups(n=8),One day before administration of drugs,rats were insulted in the forced swimming test(FST) for 15 min.On the second day,rats were injected with the same volume of saline(Group S),5 mg/kg ketamine(Group K5),10 mg/kg ketamine(Group K10),15 mg/kg(Group K15),respectively.Thirty minutes later,the locomotor activity and immobility time of rats were recorded.Experiment two: Twenty Wistar rats were randomly divided into two groups(n=10),all rats were insulted in the chronic unpredictable stress test for 21 d.On the day 22,rats were injected with the same volume of saline or ketamine 10 mg/kg,respectively.Rats horizontal movement and vertical movement were recorded at the pre-injection,and the post-injection of 1 h,2 h,6 h,1 d,4 d,7 d,respectively.Results: Compared with that in Group S,the locomotor activity in Group K5,K10 and K15 did not change(P>0.05) but the immobility time of rats(P<0.01).Compared with that in the Group saline pre-injection,the scores of horizontal movement and vertical movement(P>0.05) in group saline post-injection with 1 h,2 h,6 h,1 d,4 d and 7 d showed no significant changes;Compared with ketamine pre-injection,Group ketamine post-injection with 1 h,2 h,6 h,1 d,4 d and 7 d showed significant changes in horizontal movement and vertical movement(P<0.05).Conclusion: Ketamine has exert fast-acting and enduring antidepressant effect on depressed rat model.