ω-3 多不饱和脂肪酸对PTSD-SPS 大鼠行为学影响的研究

目的:观察饲料中添加ω-3PUFAs对PTSD-SPS大鼠焦虑/抑郁行为的防护作用。方法:将40只健康成年雄性SD大鼠随机分为正常对照组、PTSD-SPS模型组、60%ω-3PUFAs+PTSD-SPS模型组1、60%ω-3PUFAs+PTSD-SPS模型组2。采用高架十字迷宫实验和旷场实验评价实验组大鼠的焦虑/抑郁行为变化。结果:与对照组相比,SPS模型组大鼠进入开放臂的次数比例和时间比例明显减少;中央格停留时间明显缩短(5.56±0.21)s,穿格次数明显减少(30.23±5.96)次,差异均显著(P<0.05)。与SPS模型组相比,60%ω-3PUFAs的SPS组大鼠进入开放臂的次数比例和时间比例明显增加;中央格停留时间明显延长(9.88±1.14)s,穿格次数明显增加(43.22±4.35)次,差异均显著(P<0.05);与对照组相比没有显著差异。结论:膳食补充ω-3多不饱和脂肪酸可以降低PTSD-SPS大鼠焦虑/抑郁程度。

Effects of w-3 Polyunsaturated Fatty Acids on Behavioral Changes of Rats with PTSD-SPS

Objective: To investigate the effects of w-3PUFAs on the anxiety and depression-like behaviors of rats with PTSD-SPS.Methods: Forty male SD rats were randomly and equally divided into 4 groups: control group,PTSD-SPS group,60 % w-3PUFAs + PTSD-SPS group 1,60 % w-3PUFAs + PTSD-SPS group 2.The behavioral changes were evaluated by the EPM test sys-tem and the OF test system.Results: In the EPM test,the number of entering the open-arm and the open-arm detention time in the PTSD-SPS group were significantly lower than that of the control group(P<0.05).The time of resting on the center lattice and times of spanning lattice in the SPS group decreased(5.56 ± 0.21) s and(30.23 ± 5.96) times in the OF test(P<0.05),respectively.The num-ber of entering the open-arm and the open-arm detention time in the w-3PUFAs group were significantly lower than that of the SPS group(P<0.05) in the EPM test.The time of resting on the center lattice and times of spanning lattice were increased(9.88 ± 1.14) s and(43.22 ±4.35) times in the OF test(P<0.05),respectively.There were no significance difference between the w-3PUFAs group and the control group.Conclusion: Dietary Supplement of w-3PUFAs could reduce the anxiety and depression-like behaviors of PTSD-SPS model rats.