HLRP 蛋白在大鼠脑中的定位及LPS 对其表达的影响

目的:研究HLRP分子在大鼠脑中的细胞定位和表达特点,观察LPS刺激对动物脑HLRP表达的影响。方法:对原代培养的大鼠神经元、星形胶质细胞、小胶质细胞和大鼠脑组织冰冻切片分别进行免疫荧光染色,观察HLRP的细胞定位和表达特点;给大鼠侧脑室注射LPS,提脑组织蛋白,进行Western blot检测,半定量分析LPS刺激后,大鼠脑HLRP的表达变化。结果:①HLRP选择性表达于部分神经元的细胞核中,正常的星形胶质细胞和小胶质细胞不表达HLRP。②从嗅脑到脑干各节段,HLRP在大鼠脑组织中均匀分布,未发现HLRP阳性神经元聚集的现象。③侧脑室注射LPS 1天以后,HLRP表达明显升高(P<0.05)。结论:大鼠脑中正常表达HLRP,侧脑室注射LPS能刺激HLRP表达。

Distribution of HLRP in Rat Brain and Effects of LPS Lateral VentricleInjection on HLRP Expression

Objective: To investigate the location of HLRP on different cells in rat brain,and the effect of LPS lateral ventricle injection on HLRP expression.Methods: Neurons,astrocytes,and microglias were cultured seperately,and identified with anti-Tuj1,anti-GFAP,anti-OX42 IgG respectively.Then the three types of cells were co-labled with anti-HLRP IgG to investigate the location of HLRP.Discontinous frozen rat brain slices were gathered from olfactory brain to brainstem,then incubated with anti-HLRP IgG to observe the expression of HLRP.LPS solution was injected into rat lateral ventricle,and the expression of HLRP was analyzed with Westernblot method.Results: ① There was no HLRP expression in the neucleus of a certain subset of neurons,and astrocytes and microglias in normal conditions.② HLRP expression in rat brain showed no difference from olfactory brain to brainstem,and no HLRP positive neuron clusters were observed.③ After the injection LPS solution to rat lateral ventricle,HLRP expression increased obviously 1d later(P<0.05).Conclusion: HLRP expressed at normal rat neurons,and HLRP expression increased after injection LPS solution to rat lateral ventricel.