bFGF 诱导肺腺癌细胞系A549 上皮间质转化

目的:探讨上皮间质转化(epithelial-mesenchymal transition,EMT)过程在肺癌侵袭转移中的作用。方法:体外培养A549细胞,以bFGF(10ng/ml)进行干预后,倒置相差显微镜观察细胞形态学变化;间接免疫荧光观察上皮细胞标志物E-cadherin和间质细胞标志物vimentin蛋白表达的变化;采用细胞划痕试验检测bFGF对A549细胞迁移能力的影响;采用transwell小室试验检测bFGF对A549细胞侵袭能力的影响。结果:bFGF(10ng/ml)干预后,在倒置相差显微镜下观察,A549细胞形态变成了梭形,形态如同成纤维细胞。间接免疫荧光显示A549细胞E-cadherin表达随时间延长逐渐减弱,而vimentin表达逐渐增强。细胞划痕试验显示,bFGF干预后细胞迁移能力提高。Transwell小室试验显示,bFGF干预后细胞侵袭能力提高。结论:bFGF在体外诱导肺腺癌细胞系A549细胞发生上皮间质转化,上皮间质转化是肺癌侵袭转移的重要机制之一。

bFGF Induced Epithelial-Mesenchymal Transition of Human LungAdenocarcinoma Cell Line A549

Objective: To investigate the effect of epithelial to mesenchymal transition(EMT) on the invasion and metastasis of lung cancer.Methods: A549 cells cultured in vitro were treated by bFGF(10ng/ml),then the morphological changes were detected by inverted phase contrast microscopy;The expression of the epithelial marker E-cadherin and mesenchymal marker vimentin was detected by Indirect immunofluorescence;The effect of bFGF on migration of A549 was detected by scarification assay;The effect of bFGF on invasion of A549 was detected by transwell assay.Results: After treated by bFGF(10ng/ml),A549 cells turn from pebble shape to fusiform shape,a myofibroblast-like morphology.The expression of E-cadherin decreased,and the expression of vimentin increased.Scarification test showed that the migration potential of A549 cells was significantly increased after bFGF treatment.The invasive capacity of A549 increased after bFGF treatment.Conclusion: bFGF may contribute to EMT in lung adenocarcinoma cell line A549.EMT is an important mechanism for invasion and metastasis of lung cancer.