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咸宁桂花精油的前驱脂肪细胞生长和人体舒压试验的研究
引用本文:胡春芳,白红进 赵文辉 李发芳.咸宁桂花精油的前驱脂肪细胞生长和人体舒压试验的研究[J].现代生物医学进展,2012,12(13):2478-2482.
作者姓名:胡春芳  白红进 赵文辉 李发芳
作者单位:1. 塔里木大学生命科学学院 新疆阿拉尔843300;咸宁学院化学与生物学院 湖北咸宁437100
2. 塔里木大学生命科学学院 新疆阿拉尔843300
3. 咸宁市中心医院检验科 湖北咸宁437100
4. 咸宁学院化学与生物学院 湖北咸宁437100
基金项目:湖北省教育高校产学研合作资助项目(C2010064)
摘    要:本文采用超临界CO2流体法萃取三个品种咸宁桂花的干桂花得到其精油;采用石油醚浸提法浸提鲜桂花得到其浸膏,用气质联用分析了萃取桂花精油和浸膏的香气成分及其相对含量。进一步探讨了获得的精油对前驱脂肪细胞株3T3-L1细胞培养及生长的影响,并行人体神经舒压试验。结果发现其可减少细胞中的中性脂肪量;心电图LF和HF数据显示桂花精油具有一定的神经舒缓功效。

关 键 词:咸宁桂花精油  超临界CO2流体萃取  气质联用  前驱脂肪细胞  心电图

Studies on the Effect of the Osmanthus Fragrans in Xianning on the Growth of 3T3-L1 Preadipocyte and the Human Nervous Diastolic Pressure*
HU Chun-fang,BAI Hong-jin,ZHAO Wen-hui,LI Fa-fang.Studies on the Effect of the Osmanthus Fragrans in Xianning on the Growth of 3T3-L1 Preadipocyte and the Human Nervous Diastolic Pressure*[J].Progress in Modern Biomedicine,2012,12(13):2478-2482.
Authors:HU Chun-fang  BAI Hong-jin  ZHAO Wen-hui  LI Fa-fang
Institution:1 Tarim University Life sciences college,Xianjing Alaer,843300 China; 2 School of Chemistry and Life Science,Xianning University,Hubei Xianning 437100 China; 3 Clinic laboratory,Xianning Center Hospital,Hubei Xianning 437100 China)
Abstract:We extracted and got the essential oils from three varieties of dry Osmanthus fragrans in Xianning by supercritical CO2 fluid method;we extracted fresh O.fragrans by petroleum ether extraction method and got its extractum.Then we assayed by using GC-MS the fragrance ingredients and their relative content of extracted essential oils and extracts of sweet-scented O.fragrans.In this study,we performed the culture and growth experiments of 3T3-L1 preadipocyte with the O.fragrans essential oils,as well as the human nervous diastolic pressure test.Results showed the essential oils can reduce the amount of neutral fat.The data of electrocardiogram LF and HF showed the osmanthus essential oil has some nerve soothing effect.
Keywords:Osmanthus fragrans in Xianning  Supercritical CO2 fluid extraction  GC-MS  3T3-L1 preadipocyte  Electrocardiogram
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