刺五加胶囊改善抑郁大鼠学习记忆能力及对海马BDNF 表达的影响

目的:探讨刺五加胶囊对抑郁大鼠学习记忆能力及对海马BDNF表达的影响。方法:SD大鼠随机分为正常组、模型组和刺五加胶囊低、中、高剂量组,21d慢性轻度不可预见性应激刺激法(CUMS)制备大鼠抑郁模型,对照组给予生理盐水1ml灌胃,刺五加胶囊低、中、高剂量组分别给予刺五加胶囊(200mg/kg,400mg/kg,800mg/kg)灌胃,取大鼠海马组织。分别采用Morris水迷宫法和免疫组织化学法观察大鼠大鼠学习记忆能力及对海马脑源性神经生长因子(BDNF)表达。结果:刺五加胶囊低剂量组能升高海马组织中BDNF的表达(P<0.05,P<0.01),降低大鼠逃避潜伏期(EL)时间,提高大鼠空间探索时间(SET)(P<0.05,P<0.01)。结论:刺五加胶囊能升高抑郁大鼠海马组织BDNF的表达,提升抑郁大鼠学习记忆能力。

The Effects of Acanthopanacis Senticosi Pill on the Expression of TH and TPH in Hippocamp of Depressed Rats

Objective: To investigate the effects of acanthopanacis senticosi pill on the expression of BDNF in hippocamp and learning and memory capability of depressed rats.Methods: Sprague Dawley rats were divided into normal,control and acanthopanacis senticosi pill(200mg/kg,400 mg/kg,800 mg/kg)groups.Contents of BDNF in hippocamp were detected by immunity histochemical method methods,The learning and memory capability of depressed rats were measured by Morris water maze.Results: In acanthopanacis senticosi pill groups,BDNF in hippocamp and SET were increased markedly,EL were decreased markedly.Conclusion: Acanthopanacis senticosi pill could increase the expression of BDNF in hippocamp of depressed rats.It could increase learning and memory capability of depressed rats,too.