羊水干细胞治疗维甲酸诱导的大鼠骨质疏松的初步研究

目的:维甲酸(ratinoicacid)诱导雌性大鼠骨质疏松(Ostoporosis,OP)模型,观察并分析羊水干细胞(amniotic fluid-derivedstem cells,AFS)对OP的治疗作用。方法:选取40只S D雌性大鼠,随机分为4组,每组10只。除正常组(A组)外,模型组(B组),一次注入AFS组(C组)和两次注入AFS组(D组)均用维甲酸70 mg/(k g.d)连续灌胃1 4 d,A组用等量灭菌注射用水灌胃。于给药二,三周分别测量各组大鼠血清Ca、Pi、碱性磷酸酶ALP和抗酒石酸酸性磷酸酶TRACP含量,制造模型(简称造模)过程观察动物生活习性体重等变化,实验结束进行股骨骨形态计量学测量,骨薄片病理学观察。结果:造模过程中,大鼠出现少食,竖毛,及体重减轻。B.C组大鼠体重较正常组显著降低(P<0.05)。造模21d后,B组血清Ca2+水平显著升高。模型组骨密度较正常组显著下降(P<0.05),但D组骨密度变化无明显统计学意义。B,C,D组血清ALP,TRACP均明显高于正常组(P<0.05)。骨薄片染色显示模型组骨胶原排列杂乱无序,结构松散,D组有修复迹象。结论:羊水干细胞尾静脉注射可缓解维甲酸诱导的大鼠骨质疏松,且两次次给药的治疗效果好于单次给药。

Primary Study on the Effect of AFS in Ratinoicacid-Induced Ostoporosis in Rat

Objective: To observe the therapeutic effect of amniotic fluid-derived stem cells(AFS) on ratinoicacid(RA) induced Ostoporosis.Methods: Forty SD female rat were divided randomly into four groups: control group(group A),model group(group B),RA+AFS(once) group(group C),RA+AFS(twice) group(group D).Model group and treatment groups were administrated RA q.d intra-gastrically to induce OP,with control group administrated with equal volume of normal saline instead.Group C were injected with AFS through caudal vein on the 7th,14th day after first RA administration,while Group D were injected on the 14th day.On the 2nd and 3rd week of the experiment,the peripheral blood was obtained to quantify Ca2+,P,ALP and TRACP.At the end of the experient,the mea-surement of femur morphometry was carried out,then the rat were sacrificed and the femur were harvested for immunohistochemical staining and pathological examination.Results: The body weight of group C and group D were decreased markedly(P<0.05,vs group A).The concentration of Ca2+ were increased apparently(P<0.05,vs group A) and the bone density of group B was lower than group A(P<0.05),while there was no difference statistically in group D.The concentration of ALP,TRACP were much higher in model group and treatment groups than in group A.Examination of femur showed osseous collagen were deranged and surface loosening.Meanwhile,there was indication of repair in group D.Conclusion: RA-induced Ostoporosis can be relieved by injecting with AFS through caudal vein,and the prognosis of single injection was better than that of double.