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Development of twelve polymorphic microsatellite loci in polyploid endangered Omphalogramma vincaeflora Franch. (Primulaceae) |
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| Authors: | Yuan Huang Yong Li Xiao Hu Xue-Jun Ge Chang-Qin Zhang Chun-Lin Long |
| Institution: | (1) Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, The Chinese Academy of Sciences, Kunming, 650204, China;(2) School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China;(3) South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China |
| Abstract: | A total of twelve polymorphic microsatellite loci were developed from polyploid endangered species, Omphalogramma vincaeflora (Primulaceae). These loci were screened for variability among 45 individuals from three populations in China. The primers amplified loci with allele number ranging from 3 to 9, with an average of 4.25 per locus. Polymorphism information content ranged from 0.23 to 0.86. Nei’s genetic diversity ranged from 0.34 to 0.86. These primers provide an opportunity to use polymorphic DNA markers to study the population genetic structure and its breeding system in this species. |
| Keywords: | Microsatellite Omphalogramma vincaeflora Polyploidy Genetic structure Breeding system |
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