Electrogenerated chemiluminescence ethanol biosensor based on alcohol dehydrogenase functionalized Ru(bpy)3 doped silica nanoparticles |
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Authors: | Tian-tian Jia Zhi-min Cai Xiao-mei Chen Zhi-jie Lin Xiao-li Huang Xi Chen Guo-nan Chen |
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Affiliation: | aDepartment of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;bState Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China;cDepartment of Chemistry and Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China |
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Abstract: | An ethanol biosensor, based on the electrogenerated chemiluminescence of Ru(bpy)32+-doped silica nanoparticles (RuSiNPs), was investigated in this study. The biosensor was a modified glassy carbon electrode, where alcohol dehydrogenase was crosslinked to RuSiNPs, and then immobilized on the electrode surface using chitosan. The results indicated that the biosensor exhibited excellent performance during ethanol determination with a wide linear range (10−7 to 10−2 M), low detection limit (5.0 × 10−8 M) and good stability. |
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Keywords: | Electrogenerated chemiluminescence Alcohol dehydrogenase Biosensor Nanoparticles |
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