A Zea mays GTP-binding protein of the ARF family complements an Escherichia coli mutant with a temperature-sensitive malonyl-coenzyme A:acyl carrier protein transacylase |
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| Authors: | Ira I. G. S. Verwoert Adrian Brown Antoni R. Slabas Antoine R. Stuitje |
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| Affiliation: | (1) Department of Genetics, Institute for Molecular Biological Sciences, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, Netherlands;(2) Lipid Molecular Biology Group, Department of Biological Sciences, University of Durham, South Road, DH1 Durham, UK |
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| Abstract: | In an attempt to isolate a plant malonyl-coenzyme A:acyl carrier protein transacylase cDNA clone, by direct genetic selection in an Escherichia coli fabD mutant (LA2-89) with a maize cDNA expression library, a Zea mays cDNA clone encoding a GTP-binding protein of the ARF family was isolated. Complementation of a mutation affecting bacterial membrane lipid biosynthesis by a plant ARF protein, could indicate the existence of as yet unidentified bacterial equivalents of this ubiquitous eucaryotic GTP-binding protein. |
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| Keywords: | GTP binding ADP ribosylation Zea mays Escherichia coli fatty acid biosynthesis |
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