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不同种竹笋及笋壳提取物抗氧化活性的研究
引用本文:武静文,袁艺,王文文,袁彩红. 不同种竹笋及笋壳提取物抗氧化活性的研究[J]. 激光生物学报, 2011, 20(2): 245-249,260. DOI: 10.3969/j.issn.1007-7146.2011.02.019
作者姓名:武静文  袁艺  王文文  袁彩红
作者单位:安徽农业大学生命科学学院,安徽,合肥,230036
基金项目:"十一五"国家科技支撑项目
摘    要:采用醇提法,分别对毛竹、麻竹、雷竹的竹笋及笋壳进行醇提,并对醇提物进行抗氧化活性测定.对DPPH·、OH·、O2-·自由基的清除作用分别通过分光光度法、邻二氮菲法、邻苯三酚自氧化法进行测定,相对还原能力、总抗氧化能力的测定则采用普兰士蓝反应法和FRAP法.结果表明:不同种的竹笋及笋壳的醇提物含量与自由基清除能力、相对还...

关 键 词:竹笋  笋壳  DPPH·  FRAP  OH·  O2-·  相对还原能力

Antioxidant Activity of Crude Extracts from Shoots and Shells of Different Bamboos
WU Jing-wen,YUAN Yi,WANG Wen-wen,YUAN Cai-hong. Antioxidant Activity of Crude Extracts from Shoots and Shells of Different Bamboos[J]. ACTA Laser Biology Sinica, 2011, 20(2): 245-249,260. DOI: 10.3969/j.issn.1007-7146.2011.02.019
Authors:WU Jing-wen  YUAN Yi  WANG Wen-wen  YUAN Cai-hong
Affiliation:WU Jing-wen,YUAN Yi~*,WANG Wen-wen,YUAN Cai-hong (School of Life Sciences,Anhui Agriculture University,Hefei 230036,Anhui,China)
Abstract:The total oxidation resistance of ethanol extracts were determined from shoots and shells of Phyllostachys pubescens, Phyllostachys praecox and Deltdrocalamus latiflorus. The elimination of DPPH·、OH· and O2^- · were determined through spectrophotometry, phenanthroline spectrophotometry and pyrogallol autoxidation. The comparative reducing power was determined by Prussian blue reaction, and the total oxidation resistance was based on FRAP assay. The results showed that the content of crude extracts was positively related with the elimination of DPPH · , the elimination of OH · , the elimination of O2^-, comparative reducing power and total oxidation resistance. The results of antioxidant activity of crude extracts showed that PhyUostachys pubescens was the best among three kinds of bamboos, and Phyllostachys praecox was better than Dendrocalamtts latiflorus. In comparision to the winter, the antioxidant activity of Phyllostachys pubescens was better in the spring than those in th winter.
Keywords:FRAP
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