| 一株毒死蜱降解新菌株Sphingopyxis terrae R17的分离鉴定及降解特性 |
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| 引用本文： | 李瑞雪,花日茂,唐欣昀,王昀璐,张君,王道胜. 一株毒死蜱降解新菌株Sphingopyxis terrae R17的分离鉴定及降解特性[J]. 激光生物学报, 2011, 20(2): 261-268. DOI: 10.3969/j.issn.1007-7146.2011.02.022 |
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| 作者姓名： | 李瑞雪 花日茂 唐欣昀 王昀璐 张君 王道胜 |
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| 作者单位： | 1. 安徽农业大学资源与环境学院,安徽省"农产品安全"重点实验室,安徽,合肥,230036
2. 安徽农业大学生命科学学院,安徽,合肥,230036 |
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| 基金项目： | 国家"863"高技术研究发展计划项目，安徽省科技攻关项目，国家科技支撑计划项目 |
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| 摘 要： | 从农药厂排污沟污泥中分离到一株能降解毒死蜱的新菌株,命名为R17,经生理生化和16S rDNA序列同源性分析,鉴定为Sphingopyxis terrae.R17可以利用毒死蜱作为唯一碳源生长,该菌株的最适生长温度为35℃、最适pH为7～8,在此条件下培养28 h后,菌落浓度达9.18×108cfu/mL.研究了该菌在...
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| 关 键 词： | 毒死蜱 Sphingopyxis terrae 分离鉴定 降解特性 |
Isolation, Identification and Chlorpyrifos Degradation Characteristic of New Chlorpyrifos Degrading Strain Sphingopyxis terrae R17 |
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| LI Rui-xue,HUA Ri-mao,TANG Xin-yun,WANG Yun-lu,ZHANG Jun,WANG Dao-sheng. Isolation, Identification and Chlorpyrifos Degradation Characteristic of New Chlorpyrifos Degrading Strain Sphingopyxis terrae R17[J]. ACTA Laser Biology Sinica, 2011, 20(2): 261-268. DOI: 10.3969/j.issn.1007-7146.2011.02.022 |
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| Authors: | LI Rui-xue HUA Ri-mao TANG Xin-yun WANG Yun-lu ZHANG Jun WANG Dao-sheng |
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| Affiliation: | LI Rui-xue~a,HUA Ri-mao~(a*),TANG Xin-yun~(b*),WANG Yun-lu~a,ZHANG Jun~b,WANG Dao-sheng~b (Anhui Agricultural University a.College of Resources and Environment,the Key Laboratory of Anhui Agri-food Safety,Hefei 230036,Anhui,China,b.College of Life Science,China) |
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| Abstract: | One bacterial strain, named R17, was isolated from the sludge polluted with chlorpyrifos which had the capability to utilize chlorpyrifos as the sole carbon and energy sources. Strain R17 was identified preliminarily as Sphingopyxis terrae based on its physiological and biochemical characters and its 16S rDNA homologue sequence analysis. Under the optimal growth condition, 35 ℃and pH 7 - 8. Strain R17 could reach 9.18× 10^8 cfu/mL after 28 h. The chlorpyrifos degradation characteristics of strain R17 were studied with different times and different initial chlorpyrifos concentrations. The results showed that with the inoculation of 9. 18 × 10^7 cfu/mL, the degradation rate of chlorpyrifos by strain R17 were 18.59 % , 31.23 % , 36.55 % and 47.69 % in 1 d, 2 d, 3 d and4 d respectively. The degradation rate of R17 with initial concentration of 1 mg/L, 5 mg/L, 10 mg/L chlorpyrifos reached 50.21% , 43.46 % and 31.23 % after 2 d, respectively. |
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| Keywords: | Sphingopyxis terrae |
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