| 家蚕肠道产淀粉酶细菌的分离鉴定及其酶基因的克隆与表达 |
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| 引用本文： | 杨文静,王在贵,刘朝良,魏国清,朱保建,邹昌瑞. 家蚕肠道产淀粉酶细菌的分离鉴定及其酶基因的克隆与表达[J]. 激光生物学报, 2011, 20(2): 219-229. DOI: 10.3969/j.issn.1007-7146.2011.02.016 |
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| 作者姓名： | 杨文静 王在贵 刘朝良 魏国清 朱保建 邹昌瑞 |
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| 作者单位： | 安徽农业大学生命科学学院,安徽,合肥,230036 |
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| 基金项目： | 安徽省自然科学基金项目，现代农业产业技术体系建设专项资金;安徽省人才专项基金项目 |
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| 摘 要： | 从家蚕(Bombyx mori L.)5龄幼虫肠道分离鉴定产淀粉酶细菌菌株以用作微生态制剂的研究,并对该菌α-淀粉酶基因进行克隆、序列分析及在大肠杆菌中原核表达.通过含淀粉NA培养基筛选分离得到产淀粉酶菌,通过形态学观察及16S rDNA序列分析鉴定其种属,DNS法测定酶活,并设计了淀粉酶基因引物进行克隆,构建了DZ-...
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| 关 键 词： | 家蚕 肠道细菌 α-淀粉酶 16S rDNA 克隆及原核表达 |
Isolation and Identification of the Amylase-producing Bacteria in Silkworm Intestine and Cloning and Expression of Their Amylase Genes |
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| YANG Wen-jing,WANG Zai-gui,LIU Chao-liang,WEI Guo-qing,ZHU Bao-jian,ZOU Chang-rui. Isolation and Identification of the Amylase-producing Bacteria in Silkworm Intestine and Cloning and Expression of Their Amylase Genes[J]. ACTA Laser Biology Sinica, 2011, 20(2): 219-229. DOI: 10.3969/j.issn.1007-7146.2011.02.016 |
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| Authors: | YANG Wen-jing WANG Zai-gui LIU Chao-liang WEI Guo-qing ZHU Bao-jian ZOU Chang-rui |
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| Affiliation: | (College of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, China) |
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| Abstract: | Amylase-producing bacterias in larval guts of the Bombyx mori L.at 5 th year phase were isolated and identified to application for probiotics.Cloning,sequences analysis and prokaryotic expression in Escherichia coli of this amylase gene was conducted.NA starch medium was used to isolate amylase-producing bacterias.Strains identification which was on 16S rDNA sequences analysis and morphology was carried out.Enzymatic activity of them was measured by DNS method.Primers of amylase genes were designed to clone... |
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| Keywords: | 16S rDNA |
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