The Influence of Cultivation Conditions on the Proteomic Profile of <Emphasis Type="Italic">Mycobacterium tuberculosis</Emphasis> H37Rv |
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| Authors: | J A Bespyatykh O A Manicheva A V Smolyakov M Z Dogonadze V Yu Zhuravlev E A Shitikov E N Ilina |
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| Institution: | 1.Federal Research and Clinical Centre of Physical-Chemical Medicine,Moscow,Russia;2.Research Institute of Phtisiopulmonology,St. Petersburg,Russia;3.Moscow Institute of Physics and Technology,Dolgoprudny,Russia |
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| Abstract: | Comparative proteomic profiling of M. tuberculosis H37Rv strains cultured on two different nutrient media, Lowenstein-Jensen and Middlebrook 7H11, was performed using a label-free LC-MS/MS approach. It was shown that results obtained from two media possessed high convergence. The only difference was observed in the representation of fumarate reductase FrdB, its abundance was higher in the mycobacterial cells cultured on the Lowenstein-Jensen medium. The correlation analysis of biological repeats revealed the high convergence of the results obtained using the Middlebrook 7H11 medium. Thus, we can conclude that the use of the Middlebrook 7H11 medium is most appropriate in the scientific laboratory. |
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