Scope and limitations of rotating-frame nuclear Overhauser enhancement spectroscopy applied to oligosaccharides |
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| Authors: | J Breg D Romijn J F Vliegenthart G Strecker J Montreuil |
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| Institution: | Department of Bio-Organic Chemistry, University of Utrecht, The Netherlands. |
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| Abstract: | Two-dimensional rotating-frame nuclear Overhauser enhancement spectroscopy has been applied to milk oligosaccharides containing the blood-group determinants Lea, Led, or Leb. The results indicate a large number of dipolar interactions that are consistent with the preferred conformations described for these blood-group determinants. Despite careful experimental conditions, coherent transfer processes are not suppressed completely, allowing only a qualitative evaluation of the nuclear Overhauser data. The 1H- and 13C-n.m.r. spectra are assigned by comparison with literature data and by application of two-dimensional 13C-1H shift-correlation spectroscopy. |
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