Purification and characterization of pectinesterase produced by a strain ofAspergillus niger |
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| Authors: | Maldonado María C. Strasser de Saad Ana M. Callieri Danley |
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| Affiliation: | (1) Department of Industrial Microbiology, Faculty of Biochemistry and Pharmacy, National University of Tucumán, Tucumán, Argentina |
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| Abstract: | After an 88-fold purification, pectinesterase produced by a strain ofAspergillus niger, isolated from rotten lemons, showed the following main characteristics: maximum activity at 45°C, pH 5; Km, with pectin as substrate, 1.01 mg/L;  G*, 4750 Cal/mol. Polygalacturonic acid and methanol acted as competitive and non-competitive inhibitors, respectively. The activity of the enzyme was impaired by MgCl2 and stimulated by NaCl. |
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