Resonance Raman spectra of beta-carotene in native and modified low-density lipoprotein |
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Authors: | S P Verma J R Philippot B Bonnet J Sainte-Marie Y Moschetto D F Wallach |
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Institution: | 1. Radiobiology Division, Tufts-New England Medical Center, Boston, MA, USA;2. CNRS-ERA, INSERM UNITE 58, Montpellier, France;3. CNRS-LA, 79 USTL, Montpellier, France;4. INSERM, CTP, Lille, France |
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Abstract: | Low-density lipoproteins isolated between density 1.02 and 1.063 g/cm3 from normal fasting human plasma, show strong resonance Raman spectra due to the presence of beta-carotene. Three intense bands, at 1010, 1160 and 1530 cm-1, are assigned to the stretching vibrations of -C-CH3, = C-C = and -C = C- bonds, respectively, of beta-carotene. High-resolution spectra of the 1500-1600 cm-1 region reveal multiple features, suggesting the coexistence of several structural populations of beta-carotene. The modifications of lipoproteins with pH and temperature (30 degrees-42 degrees) change the resonance Raman spectra of beta-carotene. The specific binding of LDL at pH 7.0 by fibroblast cells is suppressed. Our experiments thus suggest that physical and chemical perturbations of plasma lipoproteins modify the lipid-protein interactions and thereby alter the configurational distribution of beta-carotene molecules within these particles. |
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Keywords: | LDL Low density lipoprotein RR Resonance Raman Apo B Apolipoprotein B |
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