Voltage-sensitive ion channel ofEscherichia coli |
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| Authors: | Anne H. Delcour Boris Martinac Julius Adler Ching Kung |
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| Affiliation: | (1) Departments of Biochemistry and Genetics, University of Wisconsin, 53706 Madison, Wisconsin;(2) Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, 53706 Madison, Wisconsin |
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| Abstract: | Summary A voltage-sensitive, cation-selective ion channel ofEscherichia coli has been reconstituted into liposomes and studied with the patch-clamp method. The single channel conductance was 91 pS in symmetric solutions of 150mm KCl. Many channels were open most of the time, with frequent brief transitions to closed levels. Multiple conducting units could close and reopen simultaneously, and this apparent cooperativity in gating was increases with depolarizing voltages. Above a voltage threshold, the channels closed irreversibly, often in groups. |
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| Keywords: | Escherichia coli outer membrane ion channel patch clamp porin |
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