半制备高效液相色谱制备5种苯乙醇苷类单体 |
| |
引用本文: | 陈文娟,李典鹏,陈月圆,黄永林,文永新.半制备高效液相色谱制备5种苯乙醇苷类单体[J].广西植物,2010,30(2):269-273. |
| |
作者姓名: | 陈文娟 李典鹏 陈月圆 黄永林 文永新 |
| |
作者单位: | 1. 广西壮族自治区中国科学院,广西植物研究所,广西,桂林,541006;广西师范大学,生命科学学院,广西,桂林,541004 2. 广西壮族自治区中国科学院,广西植物研究所,广西,桂林,541006 |
| |
基金项目: | 广西科学研究与技术开发计划应用基础研究专项项目 |
| |
摘 要: | 利用MCI柱层析和半制备型反相高效液相色谱法从牛耳朵中分离制备了5种苯乙醇苷类单体。在Eclipse XDB-C18(9.4mm×250mm,5μm)半制备柱上,以甲醇-水为流动相,流速为4mL/min,采用梯度洗脱方式,制备得到5个组分。经波谱(EI-MS、1H-NMR、13C-NMR)分析,分别鉴定为plantainoside(1)、chirito-sideC(2)、plantaninosideB(3)、plantamajoside(4)和desrhamnosylverbascoside(5)。高效液相色谱分析表明所制备5种化合物纯度均高于98%。该方法分离条件简单,高效,一次可得到5个单体,目标产物纯度较高。
|
关 键 词: | 半制备高效液相色谱 牛耳朵 苯乙醇苷类 |
Preparation of five phenylethanoid glycoside components by semi-preparative HPLC |
| |
Abstract: | A method was established for the isolation and preparation of five phenylethanoid glycoside components from Chirita eburnea using MCI column chromatography and semi-preparative HPLC.A semi-preparative Eclipse XDB-C18 (9.4 mm×250 mm,5 μm) was used in the preparation process.By gradient elution with methanol-water as the mobile phase at a flow rate of 4 mL/min,five phenylethanoid glycoside components were obtained from Chirita eburnea,and on the basis of spectral data (EI-MS,1H-NMR,13C-NMR),these compounds were identified as plantainoside(1),chiritoside C(2),plantaninoside B(3),plantamajoside(4) and desrhamnosylverbascoside(5),and the purities of these five phenylethanoid glycoside components analyzed by HPLC were all beyond of 98%.The method is efficient,simple with high purification and got 5 components for one time. |
| |
Keywords: | semi-preparative HPLC Chirita eburnea phenylethanoid glycosides |
本文献已被 CNKI 万方数据 等数据库收录! |
| 点击此处可从《广西植物》浏览原始摘要信息 |
| 点击此处可从《广西植物》下载免费的PDF全文 |
|