Some properties of 3H-diazepam displacing activity from human urine. |
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| Authors: | M Nielsen O Gredal C Braestrup |
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| Affiliation: | 1. Psychopharmacological Research Laboratory, Sct. Hans Mental Hospital, Dept. E, DK-4000 Roskilde, Denmark;1. A/S Ferrosan, 5 Sydmarken, DK-2860 Soeborg, Denmark |
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| Abstract: | A potent 3H-diazepam displacing factor (IC50 < 0.2 μg/ml) is isolated from human urine by adsorption to Chromosorb, elution with 50% ethanol, heating at pH 1, extractions into ethyl acetate and diethyl ether, and separation on a Sephadex LH20 column. The lipophilic compound has a molecular weight below 500 daltons and is inactivated by chymotrypsin or by exposure to pH > 12. The 3H-diazepam displacing material is probably present in urine as a conjugate or in a proform. |
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