Microbial metabolism of chlorosalicylates: accelerated evolution by natural genetic exchange |
| |
Authors: | Miguel Angel Rubio Karl-Heinrich Engesser Hans-Joachim Knackmuss |
| |
Institution: | (1) Abt. für Gewässerreinigungstechnik, Technische Universität Hamburg-Harburg, D-2100 Hamburg 90, Germany;(2) Gesamthochschule Wuppertal, Lehrstuhl für Chemische Mikrobiologie der Bergischen Universität, D-5600 Wuppertal 1, Germany;(3) Institut für Mikrobiologie der Universität Stuttgart, D-7000 Stuttgart 1, Germany |
| |
Abstract: | Methylsalicylate-grown cells of Pseudomonas sp. WR 401 cometabolized 3-, 4- and 5-substituted halosalicylates to the corresponding halocatechols. Further degradation was unproductive due to the presence of high levels of catechol 2,3-dioxygenase. This strain acquired the ability to utilize 3-chlorobenzoate following acquisition of genes from Pseudomonas sp. B 13 which are necessary for the assimilation of chlorocatechols. This derivative (WR 4011) was unable to use 4- or 5-chlorosalicylates. Derivatives able to use these compounds were obtained by plating WR 4011 on 5-chlorosalicylate minimal medium; one such derivative was designated WR 4016. The acquisition of this property was accompanied by concomitant loss of the methylsalicylate phenotype. During growth on 4- or 5-chlorosalicylate the typical enzymes of chlorocatechol assimilation were detected in cell free extracts, whereas catechol 2,3-dioxygenase activity was not induced. Repeated subcultivation of WR 4016 in the presence of 3-chlorosalicylate produced variants (WR 4016-1) which grew on all three isomers.Abbreviations CS
chlorosalicylate
- MS
methylsalicylate
- 3CB
3-chlorobenzoate
- nalr
nalidixin-resistant
- strr
streptomycin-resistant
- C230
catechol-2,3-dioxygenase
- C120
catechol-1,2-dioxygenase
- HMSH
2-hydroxymuconic semialdehyde hydrolase or 2-hydroxy-6-oxo-hexa-2,4-dienoic acid-hydrolase
- HMSD
2-hydroxymuconic semialdehyde dehydrogenase
- Dienlacton hydrolase
4-carboxymethylenebut-2-en-4-olide hydrolase |
| |
Keywords: | Mineralization Hybrids Chlorocatechol assimilation Pseudomonas sp B13 |
本文献已被 SpringerLink 等数据库收录! |
|