DNA deamination mediates innate immunity to retroviral infection |
| |
Authors: | Harris Reuben S Bishop Kate N Sheehy Ann M Craig Heather M Petersen-Mahrt Svend K Watt Ian N Neuberger Michael S Malim Michael H |
| |
Institution: | Medical Research Council Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, United Kingdom. rharris@mrc-lmb.cam.ac.uk |
| |
Abstract: | CEM15/APOBEC3G is a cellular protein required for resistance to infection by virion infectivity factor (Vif)-deficient human immunodeficiency virus (HIV). Here, using a murine leukemia virus (MLV)-based system, we provide evidence that CEM15/APOBEC3G is a DNA deaminase that is incorporated into virions during viral production and subsequently triggers massive deamination of deoxycytidine to deoxyuridine within the retroviral minus (first)-strand cDNA, thus providing a probable trigger for viral destruction. Furthermore, HIV Vif can protect MLV from this CEM15/APOBEC3G-dependent restriction. These findings imply that targeted DNA deamination is a major strategy of innate immunity to retroviruses and likely also contributes to the sequence variation observed in many viruses (including HIV). |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|