The Use of Cell Permeabilization for Studying the Mechanisms of Action of Angiotensin II in the Adrenal Cortex and Vascular Smooth Muscle

Abstract

Some applications of the technique of electropermeabilization to the study of the mechanisms of action of angiotensin II in two of its physiological target cells, the adrenal zona glomerulosa and the vascular smooth muscle cell, are described. The technique proved useful for characterizing the second messenger role of inositol 1,4,5-trisphosphate its metabolic pathways and the effect of Ca²⁺ on steroidogenesis.