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Redesigned pMAL expression vector for easy and fast purification of active native antimicrobial peptides |
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| Authors: | Lazar Gardijan Marija Miljkovic Mina Obradovic Branka Borovic Goran Vukotic Goran Jovanovic Milan Kojic |
| Affiliation: | 1. Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia;2. Institute of Meat Hygiene and Technology, Belgrade, Serbia;3. Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia Faculty of Biology, University of Belgrade, Belgrade, Serbia |
| Abstract: | |
| Keywords: | complete removal of tags and proteinase His6-enterokinase cleavage site improved pMAL and pQE vectors native antimicrobial peptides two-step chromatography |