Streptococcus strain C17T as a potential probiotic candidate to modulate oral health |
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Authors: | Wen Xiao Zhang Chun Ling Xiao Shu Yin Li Xiao Cui Bai He Qi Han Tian Nan Wang Biao Yang Xin Ming Li Ye Sun |
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Institution: | Key Lab of Environmental Pollution and Microecology of Liaoning Province, Shenyang Medical College, Shenyang, Liao Ning, People’s Republic of China |
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Abstract: | In the microbiome, probiotics modulate oral diseases. In this study, Streptococcus strain C17T was isolated from the oropharynx of a 5-year-old healthy child, and its potential probiotic properties were analysed using human bronchial epithelial cells (16-HBE) used as an in vitro oropharyngeal mucosal model. The results demonstrated that the C17T strain showed tolerance to moderate pH ranges of 4–5 and 0·5–1% bile. However, it was more tolerant to 0·5% bile than 1% bile. It also demonstrated an ability to accommodate maladaptive oropharyngeal conditions (i.e. tolerating lysozyme at 200 μg ml?1). It was also resistant to hydrogen peroxide at 0·8 mM. In addition, we found out that the strain possesses inhibitory activities against various common pathogenic bacteria. Furthermore, C17T was not cytotoxic to 16-HBE cells at different multiplicities of infection. Scanning electron microscopy disclosed that C17T adhesion to 16-HBE cells. Competition, exclusion and displacement assays showed that it had good anti-adhesive effect against S. aureus. The present study revealed that Streptococcus strain C17T is a potentially efficacious oropharyngeal probiotic. |
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Keywords: | adhesion to 16-HBE anti-adhesion assay antimicrobial activity probiotic Staphylococcus aureus |
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