香菇菌丝深层培养的碳、氮源和生长因子

本文分别设计了生长刺激因子、碳、氮源试验基本培养基。结果表明在深层培养时,废糖蜜、粗制蔗糖、玉米浆、酵母粉、麸皮浸汁中含有刺激香菇菌丝生长的物质。而各种维生素(包括 B_1)、氨基酸、有机酸、植物生长激素等均无明显的作用。在上述生长因子的促进下,香菇菌丝可利用无机或有机氮源。无机氮源以 NH_4NO_3,最好,其次为(NH_3)H_2PO_4、NH)4Cl 等。有机氮源以玉米浆、酵母粉最好。碳源以玉米淀粉最好。本文并介绍了一种廉价的、良好的香菇菌丝液体培养配方,可以稳定地培养出细小均匀的菌丝球、生长丰满稠密的液体菌种。

INVESTIGATIONS ON THE CARBON,NITROGEN SOURCE AND GROWTH FACTOR REQUIREMENTS FOR MYCELIAL GROWTH OF LENTINUS EDODES IN SUBMERGED CULTURE

Several basal media on carbon,nitrogen sources and growth fac- tor tests were designed for the experiments.The experimental results indicated that waste molasses,brown sugar,corn-steep liquor,yeast powder,bran extract may contain some sub- stances which could stimulate the mycclial growth of Lentinus edodes in submerged culture, while various vitamins(including thiamine),amino-acids,organic acids,plant growth hormones,etc.were shown to be without distinct effect.With the promotion of the above- mentioned substances,the mycelium of L.edodes could then utilize inorganic or organic ni- trogen sources.Among the inorganic nitrogen compounds tested,NH_4NO_3 is the most desi- rable,and(NH_4)H_2PO_4,NH_4CI,etc.Corn-steep liquor and yeast powder were found to be the most preferable substances in comparable to the other organic nitrogen.Corn starch was proved to be the most suitable carbon source.A desirable and cheap formulation of a liquid medium is described,and in such medium,a dense suspension of L.edodes with numerous small and homogeneous mycelial pellets,could be successfully produced.