Protein kinase C and leucine metabolism in intestinal crypt cells |
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| Authors: | I F Durr K Badjakian S Ismail T Shatila |
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| Institution: | 1. Gansu Provincial Key Laboratory of Wearable Computing, School of Information Science and Engineering, Lanzhou University, Gansu 730000, China;2. Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China;1. Department of Epidemiology, College for Public Health & Social Justice, Saint Louis University, 3545 Lafayette Avenue, St. Louis, MO 63130, United States;2. Department of Physical Education, School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil;3. Prevention Research Center in St. Louis, Brown School, Washington University in St. Louis, One Brookings Drive, St. Louis, MO 63130, United States;4. Health Communications Research Laboratory, Washington University in St. Louis, 700 Rosedale Avenue, St. Louis, MO 63112, United States;5. Department of Behavioral Science and Health Education, College for Public Health and Social Justice, Saint Louis University, 3545 Lafayette Ave., St Louis, MO 63130, United States;1. Department of Agriculture Biotechnology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250 110, Uttar Pradesh, India;2. Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València, Camino de Vera 14, Valencia 46022, Spain;3. Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;4. Department of Botany, GDC, Pulwama-192301, Jammu and Kashmir, India;1. Department of Cardiovascular Medicine, National Cerebral and Cardiovascular Center, Suita, Japan;2. Division of Advanced Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan;3. Office of Biostatistics and Data Management, Department of Advanced Medical Technology Development, National Cerebral and Cardiovascular Center, Suita, Japan;4. Division of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan |
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| Abstract: | Intestinal cells were separated into fractions rich in villous or crypt cells. Alkaline phosphatase, present in villous cells, but absent from crypt cells, was used as a marker. Crypt cells were 3-6 times as active as villous cells in the metabolism of leucine or mevalonate. The previously reported stimulatory effect of albumin was twice as strong in crypt cells as that in villous cells. Reduced glutathione, spermidine HCl and ethanolamine (0.5-10 mM) did not replace albumin, the effect of which was maximal at 0.02 mM. Protein kinase C was shown to be present mainly in crypt cells. |
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