The phosphoinositide phosphatase Sac1 is required for midline axon guidance |
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Authors: | Seongsoo Lee Sungdae Kim Minyeop Nahm Euijae Kim Tai-Il Kim Jin Ho Yoon Seungbok Lee |
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Institution: | (1) Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA; |
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Abstract: | Sac1 phosphoinositide (PI) phosphatases are important regulators of PtdIns(4)P turnover at the ER, Golgi, and plasma membrane
(PM) and are involved in diverse cellular processes including cytoskeletal organization and vesicular trafficking. Here, we
present evidence that Sac1 regulates axon guidance in the embryonic CNS of Drosophila. Sac1 is expressed on three longitudinal axon tracts that are defined by the cell adhesion molecule Fasciclin II (Fas II).
Mutations in the sac1 gene cause ectopic midline crossing of Fas II-positive axon tracts. This phenotype is rescued by neuronal
expression of wild-type Sac1 but not by a catalytically-inactive mutant. Finally, sac1 displays dosage-sensitive genetic interactions with mutations in the genes that encode the midline repellent Slit and its
axonal receptor Robo. Taken together, our results suggest that Sac1-mediated regulation of PIs is critical for Slit/Robo-dependent
axon repulsion at the CNS midline. |
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Keywords: | Drosophila midline axon guidance phosphoinositide phosphatase Sac1 |
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