Immunohistochemical localization of secretory proteins in the endometrial epithelium of the rabbit |
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| Authors: | Professor Christoph Kirchner |
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| Affiliation: | (1) Department of Biology, University of Marburg, Marburg a.d. Lahn, Germany;(2) Fachbereich Biologie, 3550 Lahnberge, Marburg, Germany |
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| Abstract: | Summary Proteins of uterine fluid and lung homogenates of the rabbit were separated by gel and ion exchange chromatography. Purified protein fractions were used for immunisation and antiserum production. By means of several absorptions, six monospecific antisera against uteroglobin and five other proteins were obtained. Using immunohistochemistry, four of them could be localised in the uterine epithelium from oestrus and the first and the seventh day post coitum, and also in the blastocyst. The present study indicates the involvement of different endometrial cells in the synthesis and release of the various proteins of uterine secretion.Supported by grant Ki 154/6-7 from the Deutsche Forschungsgemeinschaft |
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| Keywords: | Uterine proteins (rabbit) Uteroglobin Blastocyst Immunohistochemistry Gel chromatography Disc electrophoresis Immunoelectrophoresis |
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