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Recent advances in the application of expressed protein ligation to protein engineering
Authors:Hofmann Roseanne M  Muir Tom W
Affiliation:Laboratory of Synthetic Protein Chemistry, The Rockefeller University, New York, NY 10021, USA.
Abstract:Expressed protein ligation is a technique for joining recombinantly expressed proteins to polypeptides containing biophysical probes, post-translational modifications or unnatural amino acids. Recent advances have expanded the scope of expressed protein ligation and have allowed the approach to be applied to the study of basic biological questions.
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