Efficient protoplast regeneration ofBacillus thuringiensis andAgrobacterium tumefaciens |
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Authors: | U. S. Puntambekar D. V. Gokhale P. K. Ranjekar |
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Affiliation: | (1) Division of Biochemical Sciences, National Chemical Laboratory, 411 008 Pune, India |
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Abstract: | Summary Treatment ofBacillus thuringiensis andAgrobacterium tumefaciens taken from the early growth phase (8 h) with lysozyme at 1 mg/ml gave 90–99% protoplast formation and 10–12% protoplast regeneration on the minimal medium in absence of plasma expander (Bovine serum albumin). Enhanced fusion frequency was obtained when protoplasts from 8 h grown cells were used for fusion experiments. |
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