Abstract: | We prepared transfer factor from mononuclear (M) cells and polymorphonuclear (PMN) cells by sieving cell lysates through a P-2 Biogel column. By assaying column fractions by their ability to induce a local delayed-type reaction to antigen in canine skin, we determined that mononuclear cells most consistently contained skin activity. The elution patterns from P-2 Biogel of PMN or M-enriched preparations were less complex and much different from that seen with whole leukocytes. |