Coordinate induction of glutathione biosynthesis and glutathione-metabolizing enzymes is correlated with salt tolerance in tomato |
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| Authors: | Mittova Valentina Theodoulou Frederica L Kiddle Guy Gómez Leonardo Volokita Micha Tal Moshe Foyer Christine H Guy Micha |
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| Institution: | Albert Katz Department of Drylands Biotechnologies, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus 84990, Israel. |
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| Abstract: | The acclimation of reduced glutathione (GSH) biosynthesis and GSH-utilizing enzymes to salt stress was studied in two tomato species that differ in stress tolerance. Salt increased GSH content and GSH:GSSG (oxidized glutathione) ratio in oxidative stress-tolerant Lycopersicon pennellii (Lpa) but not in Lycopersicon esculentum (Lem). These changes were associated with salt-induced upregulation of gamma-glutamylcysteine synthetase protein, an effect which was prevented by preincubation with buthionine sulfoximine. Salt treatment induced glutathione peroxidase and glutathione-S-transferase but not glutathione reductase activities in Lpa. These results suggest a mechanism of coordinate upregulation of synthesis and metabolism of GSH in Lpa, that is absent from Lem. |
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| Keywords: | Salt tolerance Tomato Glutathione γ-Glutamylcysteine synthetase Glutathione-S-transferase Glutathione peroxidase |
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