Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, <Emphasis Type="Italic">Macrobrachium rosenbergii</Emphasis> |
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| Authors: | L M See R Hassan S G Tan S Bhassu |
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| Institution: | 1.Biology Department, Faculty of Science,University Putra Malaysia,Serdang Selangor,Malaysia;2.Freshwater Fisheries Research Center,Titi, Jelebu,Malaysia;3.Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences,University Putra Malaysia,Serdang Selangor,Malaysia;4.Department of Genetics, Institute of Biological Sciences,University Malaya,Kuala Lumpur,Malaysia;5.Center for Biotechnology in Agriculture (CEBAR),Kuala Lumpur,Malaya |
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| Abstract: | Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for
this species. The bands resulting from the PCR amplifications of these eight microsatellite loci were polymorphic with the
number of alleles ranging from 8 to 26 alleles per locus, whereas the observed heterozygosity ranged from 0.0641 to 0.6564.
These newly developed microsatellite markers should prove to be useful for population studies and in the management of genetic
variations in broodstocks of freshwater prawn, M. rosenbergii. |
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