Structural identification of oligosaccharides produced by Zymomonas mobilis levansucrase |
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| Authors: | R G Crittenden H W Doelle |
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| Institution: | (1) Department of Microbiology, University of Queensland, 4072 St Lucia, Australia |
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| Abstract: | Summary The chemical structure of oligosaccharides produced by Zymomonas mobilis levansucrase (EC 2.4.1.10) was determined using enzymatic hydrolysis, mass spectroscopy, and 13C-NMR spectroscopy. The major oligosaccharide (98% of total oligomers) produced from transfructosylation reactions with -sucrose was identified as 1-kestose (O- -D-glucopyranosyl-(1 2)-O- -D-fructofuranosyl-(12)--D-fructofuranoside). |
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