Development and interspecific transferability of genic microsatellite markers in Spartina spp. with different genome size |
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| Authors: | Niranjan Baisakh Prasanta K Subudhi K Arumuganathan Arnold P Parco Stephen A Harrison Carrie A Knott Michael D Materne |
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| Institution: | a104 M.B. Sturgis Hall, School of Plant, Environmental, and Soil Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA;bFlow and Image Cytometry Core Research Facilities, Virginia Mason Research Center, 1201 ninth Avenue, Seattle, WA 98101, USA |
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| Abstract: | Flow cytometry analysis showed variation of nuclear DNA content among different species of Spartina. Spartina alterniflora had the biggest genome (1763.9 Mbp) and S. cynosuroides had the smallest genome (756.35 Mbp), whereas the genomes of S. patens (969.36 Mbp) and S. spartinae (979.78 Mbp) were comparable. Mining simple sequence repeats (SSR) from 1227 expressed sequence tags (EST) generated from salt stressed S. alterniflora showed an abundance of di- and tri-nucleotide repeats. Of 100 ESSR (EST-derived SSR) loci with five or more repeats, 81 loci were successfully amplified in eight S. alterniflora genotypes and 15 (22.2%) ESSR markers were polymorphic. Eleven of the 15 polymorphic ESSRs showed amplification across six different species of Spartina while 100% cross transferability was observed with at least one species of Spartina. The average number of alleles per marker was 3.9 and 5.8 within S. alterniflora and among Spartina species, respectively. The ESSR markers discriminated different members within and between species of Spartina genus. |
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| Keywords: | DNA content Expressed sequence tag Polymorphism Simple sequence repeat Spartina Transferability |
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