Identification of potential CepR regulated genes using a cep box motif-based search of the Burkholderia cenocepacia genome |
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Authors: | Catherine E Chambers Erika I Lutter Michelle B Visser Peggy PY Law Pamela A Sokol |
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Institution: | (1) Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada |
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Abstract: | Background The Burkholderia cenocepacia CepIR quorum sensing system has been shown to positively and negatively regulate genes involved in siderophore production,
protease expression, motility, biofilm formation and virulence. In this study, two approaches were used to identify genes
regulated by the CepIR quorum sensing system. Transposon mutagenesis was used to create lacZ promoter fusions in a cepI mutant that were screened for differential expression in the presence of N-acylhomoserine lactones. A bioinformatics approach was used to screen the B. cenocepacia J2315 genome for CepR binding site motifs. |
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