Display of a thermostable lipase on the surface of a solvent-resistant bacterium, Pseudomonas putida GM730, and its applications in whole-cell biocatalysis |
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Authors: | Heung-Chae Jung Seok-Joon Kwon Jae-Gu Pan |
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Institution: | (1) National Research Laboratory of Microbial Display, GenoFocus, Inc., 461-58 Jeonmindong, Yusong, Daejeon, 305-811, Republic of Korea;(2) Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oundong, Yusong, Daejeon, 305-333, Republic of Korea;(3) Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590, USA |
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Abstract: | Background Whole-cell biocatalysis in organic solvents has been widely applied to industrial bioprocesses. In two-phase water-solvent
processes, substrate conversion yields and volumetric productivities can be limited by the toxicity of solvents to host cells
and by the low mass transfer rates of the substrates from the solvent phase to the whole-cell biocatalysts in water. |
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