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Display of a thermostable lipase on the surface of a solvent-resistant bacterium, Pseudomonas putida GM730, and its applications in whole-cell biocatalysis
Authors:Heung-Chae Jung  Seok-Joon Kwon  Jae-Gu Pan
Institution:(1) National Research Laboratory of Microbial Display, GenoFocus, Inc., 461-58 Jeonmindong, Yusong, Daejeon, 305-811, Republic of Korea;(2) Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oundong, Yusong, Daejeon, 305-333, Republic of Korea;(3) Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590, USA
Abstract:

Background  

Whole-cell biocatalysis in organic solvents has been widely applied to industrial bioprocesses. In two-phase water-solvent processes, substrate conversion yields and volumetric productivities can be limited by the toxicity of solvents to host cells and by the low mass transfer rates of the substrates from the solvent phase to the whole-cell biocatalysts in water.
Keywords:
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