Biochemical Changes Associated with the Growth of Root Tips of Vicia faba in vitro, and the Effect of 2,4-Dichlorophenoxyacetic Acid |
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Authors: | GRANT M E; FULLER K W |
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Abstract: | Root tips of Vicia faba were cultured for 3 weeks on a semi-solidmedium containing 40 g/l sucrose ±105 M 2:4-dichlorophenoxyaceticacid (2,4-D). Inhibition of root elongation and the stimulationof an actively dividing meristematic zone in the pericycle regionwere observed in the 2,4-D-treated tissues. Biochemical dataon the DNA content and respiration of these root tips correlatewell with the morphological observations. 2,4-D also induceda marked decrease in the -cellulose content of the cell wallsand analyses of the carbohydrate content of the ethanol-solublepool and starch content of the cultured root tips have providedtentative evidence for some of the controlling factors exertedin the presence and absence of 2,4-D. |
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