Syntaxin 1A and Receptor for Activated C Kinase Interact with the N-Terminal Region of Human Dopamine Transporter |
| |
Authors: | Lee Ki-Hwan Kim Mi-Young Kim Dong-Hwan Lee Yong-Sung |
| |
Institution: | Department of Biochemistry, College of Medicine, Hanyang University, Seoul, Korea. |
| |
Abstract: | The dopamine transporter (DAT) regulates the extent and duration of dopamine receptor activation through sodium-dependant reuptake of dopamine into presynaptic neurons, resulting in termination of dopaminergic neurotransmission. Using the yeast two-hybrid system, we have identified novel interactions between DAT, the SNARE protein syntaxin 1A, and the receptor for activated C kinases (RACK1). This association involves the intracellular N-terminal domain of human DAT (hDAT). Our data suggest that hDAT may exist as dimers or oligomers and that its protein-protein interactions with syntaxin 1A and RACK1 form functional regulatory complexes that may mediate DAT trafficking through modulation of hDAT phosphorylation by PKC. |
| |
Keywords: | Coimmunoprecipitation dopamine transporter RACK1 syntaxin 1A yeast-two-hybrid |
本文献已被 PubMed SpringerLink 等数据库收录! |