白细胞介素8 基因-251A/T 多态与子宫内膜异位症的相关性研究

目的:探讨白细胞介素8基因(IL-8)-251A/T多态性在子宫内膜异位症发生中的作用。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和焦磷酸测序方法,研究152例子宫内膜异位症患者和134例正常子宫内膜中IL-8基因-251A/T多态性的分布情况。结果:IL-8基因-251A/T多态位点的AA、AT和TT基因型在子宫内膜异位症组中分别为12.5%、46.1%、41.5%,等位基因A和T的频率分别为35.5%、64.5%;在对照组中AA、AT和TT三种基因型分布频率分别为24.6%、41.0%、34.3%,等位基因A和T的频率分别为45.1%、54.9%。IL-8基因-251A/T单核苷酸多态是子宫内膜异位症发病的独立的危险因素(P<0.05);A等位基因携带者患子宫内膜异位症的风险增高(P<0.05)。结论:在中国北方汉族人群中IL-8基因-251A/T单核苷酸多态性与子宫内膜异位症的发病具有相关性,A等位基因是子宫内膜异位症发病重要的遗传学标记。

Relationship between IL-8 Gene Polymorphisms and Endometriosis

Objective: To evaluate the effect of IL-8 gene polymorphisms in the pathogenesis of endometriosis.Methods: The distribution of IL-8 gene-251A/T polymorphisms in 152 specimens of endometriosis and 134 specimens of normal endometrium was determined by RFLP-PCR and DNA sequencing.Results: IL-8 gene-251A/T polymorphism of the AA,AT and TT genotypes in the endometriosis group was 12.5%,46.1% and 41.5%,while A and T allele frequencies were 35.5% and 64.5%,respectively.Genotypes in healthy adults were 24.6%,41.0%,34.3%,A and T allele frequencies were 45.1% and 54.9% respectively.The distribution of IL-8 gene-251 A/T polymorphism in the two groups was significantly different(P<0.05);the risk of endometriosis was markedly higher in the A allele carriers than that in the T allele carriers.Conclution: The finding suggests that there is significant correlation between IL-8 gene-251A/T polymorphisms and the pathogenesis of endometriosis.