| 大鼠SR-BI胞外域与ApoA-Ⅰ蛋白相互作用的确认 |
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| 引用本文： | 李兰松 李维琪 信学雷 曹旭. 大鼠SR-BI胞外域与ApoA-Ⅰ蛋白相互作用的确认[J]. 微生物学通报, 2006, 33(2): 68-73 |
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| 作者姓名： | 李兰松 李维琪 信学雷 曹旭 |
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| 作者单位： | [1]中国科学院新疆理化技术研究所,乌鲁木齐830011 [2]中国科学院研究生院,北京100039 |
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| 基金项目： | 中国科学院“百人计划”项目资助;2001年“西部之光”项目资助 |
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| 摘 要： | 先以含全长SR-BI cDNA序列的重组质粒pMD18-T-rS为摸板进行PCR反应扩增SR-BI得到胞外域cDNA片段，经测序证明正确后，定向克隆到酵母双杂交表达载体，然后与pGBKT7-ApoA-Ⅰ质粒共转化酵母细胞，通过报告基因及酵母交配试验确认了SR-BI的胞外域部分和ApoA-Ⅰ之间的确存，观察到ApoA-Ⅰ与SR-BI胞外域间的相互作用力比与全长的SR-BI问的相互作用力提高了10％。
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| 关 键 词： | 载脂蛋白AI（ApoA-Ⅰ） B族I型清道夫受体（SR-BI） SR-BI胞外域 基因克隆 酵母双杂交 |
| 文章编号： | 0253-2654（2006）02-0068-06 |
| 收稿时间： | 2005-06-20 |
| 修稿时间： | 2005-08-15 |
Verification the Interaction of Apolipoprotein AI and the Extracellular Domain of Scavenger Receptors Class B Type I |
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| LI Lan-Song,LI Wei-Qi,XIN Xue-Lei, CAO Xu. Verification the Interaction of Apolipoprotein AI and the Extracellular Domain of Scavenger Receptors Class B Type I[J]. Microbiology China, 2006, 33(2): 68-73 |
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| Authors: | LI Lan-Song LI Wei-Qi XIN Xue-Lei CAO Xu |
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| Abstract: | In this paper,the DNA fragment rSE was amplified by PCR using the recombinant plasmids pMD18-T-rS (containing the hole lines of cDNA of SR-BI) as template. Then the PCR product was directionally cloned into the yeast two hybrid express vector pGADT7 after it was confirmed with sequencing. The recombined vector (named pGADT7-rSE) and pGBKT7-ApoA-I vector was cotransformed in yeast strain AH109. We identified the interaction of ApoA-I and rSE by using report genes and yeast mating. At last,we obsevered that the interaction of ApoA-I and rSE is about 10% more than that of ApoA-I and SR-BI of rat. |
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| Keywords: | Apolipoprotein AI(ApoAI) Scavenger receptor class B type I(SR-BI) Extracellular domain of SR-BI Gene clone Yeast two hybrid |
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