Mechanosensitive potassium channels in locust muscle membrane |
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| Authors: | I. R. Mellor B. A. Miller A. G. Petrov I. Tabarean P. N. R. Usherwood |
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| Affiliation: | (1) Division of Molecular Toxicology, School of Biological Sciences, University of Nottingham, Nottingham NG7 2RD, UK, GB;(2) Biomolecular Layers Department, Institute of Solid State Physics, Bulgarian Academy of Sciences, BG-1784 Sofia, Bulgaria e-mail: agpetrov@issp.bas.bg, BG |
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| Abstract: | Patch clamp recordings have been made from adult locust (Schistocerca gregaria) muscle membrane to study the mechanosensitivity of potassium channels (BK and IK) in cell-attached patches by transiently applying measured pressures to the contents of the patch pipettes. The aim of the investigations was to demonstrate a novel gating behaviour by pressure of the BK channel in contrast to the familiar behaviour of the IK channel. The open probability (p 0) of the IK channel increased rapidly in response to a pressure step and monotonically during a pressure ramp. This gating was readily repeatable and rapidly reversible. The relationship between ln[p 0/(1–p 0)] and transmembrane pressure was linear. In comparison, p 0 for the BK channel was also increased by pressure, but its gating was delayed, cumulative, and hysteretic. Received: 12 July 1998 / Revised version: 7 October 1998 / Accepted: 7 October 1998 |
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| Keywords: | Mechanosensitivity Stretch sensitivity Curvature sensitivity Potassium channels Locust muscle |
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