Ritonavir does not inhibit calpain in vitro |
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Authors: | Cuerrier Dominic Nie Zilin Badley Andrew D Davies Peter L |
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Affiliation: | Department of Biochemistry, Queen's University, Kingston, Ont., Canada K7L 3N6. |
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Abstract: | Ritonavir, an inhibitor of HIV-1 protease, has been reported to also inhibit the Ca2+-dependent cysteine protease, calpain. We have investigated these claims with an in vitro study of the effect of ritonavir on the m-calpain and mu-calpain isoforms. Ritonavir failed to block either autolytic or hydrolytic calpain activity, but remained fully capable of inhibiting the HIV-1 protease. Any calpain-related effects of ritonavir in cells must, therefore, arise by a mechanism other than direct inhibition of calpains. |
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Keywords: | Calpain Protease Ritonavir Inhibitor Autolysis |
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