Effects of electric cell fusion treatment among leaf protoplasts of Populus alba and alnus firma on growth, leaf morphology, and rapd pattern of eleven acclimatized plants |
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Authors: | Hamako Sasamoto Yohichi Wakita Shinso Yokota Nobuo Yoshizawa Toshio Katsuki Yoshihiko Nishiyama Toshitaka Yokoyama Mitsue Fukui |
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Institution: | (1) Faculty of Environment and Information Sciences, Yokohama National University, 240-8501 Yokohama, Japan;(2) Greenery Research and Information Centre, Hokkaido Forestry Research Institute, 079-0198 Bibai, Hokkaido, Japan;(3) Department of Forest Science, Faculty of Agriculture, Utsunomiya University, 321-8505 Utsunomiya, Tochigi, Japan;(4) Forestry and Forest Products Research Institute, 305-8687 Tsukuba, Ibaraki, Japan |
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Abstract: | Summary This study reports the characterization of 11 plants regenerated from electrically fused protoplasts between Populus alba and Alnus firma. Growth characteristics of five regenerated plants (AP-1-AP-5) in terms of shoot height and leaf color showed small differences
compared with those of P. alba grown, in pots, and showed no difference in shoot height and diameter compared with those grown in nursery field. There was
also no difference in the RAPD pattern between the plants regenerated from interfamilial protoplast fusion and P. alba. In contrast, the lately regenerated plants (AP-6-AP-11) grown in pots showed a marked difference in leaf morphology and
RAPD pattern. There was a variation in the ratio of longitudinal to transverse length of leaves among the 11 plants from interfamilial
fusions compared with that of protoclones and intraspecific fused protoplasts of P. alba. |
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Keywords: | Betulaceae electric fusion protoplast random amplified polymorphic DNA Salicaceae somatic hybrid |
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