Isolation of thermostable D-hydantoinase-producing thermophilicBacillus sp SD-1 |
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| Authors: | Seung-Goo Lee Dong-Cheol Lee Moon-Hee Sung Hak-Sung Kim |
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| Affiliation: | (1) Department of Biotechnology, Korea Advanced Institute of Science and Technology, 373-1 Kusung-Dong, Yusung-Gu, 305-701 Taejon, Korea;(2) Applied Microbiology Research Group, GERI, KIST, Yusung-Gu, P.O. Box 115, 305-600 Taejon, Korea |
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| Abstract: | Summary A thermophilic bacterium which showed highest thermostability and activity of the hydantoinase was isolated from 1m000 thermophiles and identified to beBacillus sp. SD-1 according to morphological and physiological characteristics. The optimal growth temperature of the bacterium was about 60°C. The hydantoinase ofBacillus sp. SD-1 was strictly D-specific, and optimal pH and temperature were determined to be about 8.0 and 70°C, respectively. The D-hydantoinase was stable upto 70°C, and half-life of the enzyme was about 20 min at 80°C. |
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