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Expression and functional analysis of an Entamoeba histolytica truncated adhesin in tomato plants |
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| Authors: | Felipe Guadalupe Carrillo Landell Guillermina García Rivera Teresa de Jesús Olivera Flores Máximo B. Martínez Esther Orozco Orozco Miguel Angel Gómez Lim |
| Affiliation: | (1) Departamento de Ingeniería Genética de Plantas, Cinvestav Campus Guanajuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, Apdo. Postal 629, Irapuato, Mexico, 36500, Guanajuato, Mexico;(2) Departamento de Patología Experimental, Cinvestav, Av. I.P.N. 2508. Col. San Pedro Zacatenco, Mexico, DF, 07360, Mexico;(3) Facultad de Química, Conjunto E, Laboratorio 116, Paseo de la Investigación Científica, Universidad Nacional Autónoma de México, Mexico, DF, CP 04510, Mexico |
| Abstract: | Several antigens from Entamoeba histolytica the agent causing human amoebiasis have been identified as potential vaccine candidates. One of them is the EhADH112 adhesin, which plays an important role during trophozoite adherence to and phagocytosis of target cells. We report here the expression of the EhADH112 carboxy-terminus of the gene (Adh240) in tomato plants. A 28 kDa band is recognized by specific antibodies in total proteins from transformed tomatoes. The plant-based adhesin conserves the ability to inhibit trophozoite adherence and phagocytosis of target cells by an average of 32.8 and 44.75% respectively. |
| Keywords: | Edible vaccines Transgenic plants Tomato Entamoeba histolytica Amoebiasis |
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