Cultivar Identification and Genetic Diversity of Chinese Bayberry (<Emphasis Type="Italic">Myrica rubra</Emphasis>) Accessions Based on Fluorescent SSR Markers |
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Authors: | Rang-Jin Xie Jian Zhou Guo-Yun Wang Shui-Ming Zhang Lin Chen Zhong-Shan Gao |
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Institution: | (1) Department of Horticulture, Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, State Agriculture Ministry, Zhejiang University, Hangzhou, 310029, People’s Republic of China;(2) Citrus Research Institute, Southwest University, Chongqing, 400712, People’s Republic of China;(3) Fruit Research Institute, Yuyao Ningbo, 315400, People’s Republic of China;(4) Department of Horticulture, Anhui Agricultural University, Hefei, 230036, People’s Republic of China; |
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Abstract: | A collection of 122 Chinese bayberry accessions and one wax myrtle (Myrica cerifera L.) were analyzed with 14 polymorphic simple sequence repeats (SSRs). The average number of alleles per locus was 9.3, and
polymorphism information content varied from 0.07 to 0.83, with a mean value of 0.62. The genetic relationships among the
123 accessions were analyzed using the unweighted pair-group method with arithmetic mean (UPGMA). The similarity among all
the accessions, based on Dice’s coefficient, varied from 0.78 to 0.99, and 0.74 between the Chinese bayberries and wax myrtle.
A set of 122 Chinese bayberries clustered into four groups, with the first group further divided into six subgroups. The accessions
originating from the same geographical region were more closely related than those from different regions, although extensive
gene flow has taken place. The Mantel test, used to compare similarity matrices calculated from AFLP and SSR data, showed
that their combination could provide information on the genetic relationship among the Chinese bayberry accessions. Ten selected
SSR markers were able to distinguish most accessions, and multiplex PCR systems were developed. In addition, we found that
SSRs developed from Chinese bayberry are transferable to M. cerifera. |
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